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    A comparison of labeling and label-free Mass Spectrometry-based Proteomics approaches

    Patel, V.J. and Thalassinos, Konstantinos and Slade, S.E. and Connolly, J.B. and Crombie, A. and Murrell, J.C. and Scrivens, J.H. (2009) A comparison of labeling and label-free Mass Spectrometry-based Proteomics approaches. Journal of Proteome Research 8 (7), pp. 3752-3759. ISSN 1535-3893.

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    Abstract

    The proteome of the recently discovered bacterium Methylocella silvestris has been characterized using three profiling and comparative proteomics approaches. The organism has been grown on two different substrates enabling variations in protein expression to be identified. The results obtained using the experimental approaches have been compared with respect to number of proteins identified, confidence in identification, sequence coverage and agreement of regulated proteins. The sample preparation, instrumental time and sample loading requirements of the differing experiments are compared and discussed. A preliminary screen of the protein regulation results for biological significance has also been performed.

    Metadata

    Item Type: Article
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 20 May 2013 11:38
    Last Modified: 06 Dec 2016 10:40
    URI: http://eprints.bbk.ac.uk/id/eprint/6894

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