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    Crystal structure of the haemophilus influenzae hap adhesin reveals an intercellular oligomerization mechanism for bacterial aggregation

    Meng, G. and Spahich, N. and Kenjale, R. and Waksman, Gabriel and St Geme, J.W. (2011) Crystal structure of the haemophilus influenzae hap adhesin reveals an intercellular oligomerization mechanism for bacterial aggregation. The EMBO Journal 30 (18), pp. 3663-3874. ISSN 0261-4189.

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    Abstract

    Bacterial biofilms are complex microbial communities that are common in nature and are being recognized increasingly as an important determinant of bacterial virulence. However, the structural determinants of bacterial aggregation and eventual biofilm formation have been poorly defined. In Gram-negative bacteria, a major subgroup of extracellular proteins called self-associating autotransporters (SAATs) can mediate cell-cell adhesion and facilitate biofilm formation. In this study, we used the Haemophilus influenzae Hap autotransporter as a prototype SAAT to understand how bacteria associate with each other. The crystal structure of the H. influenzae Hap(S) passenger domain (harbouring the SAAT domain) was determined to 2.2 Å by X-ray crystallography, revealing an unprecedented intercellular oligomerization mechanism for cell-cell interaction. The C-terminal SAAT domain folds into a triangular-prism-like structure that can mediate Hap-Hap dimerization and higher degrees of multimerization through its F1-F2 edge and F2 face. The intercellular multimerization can give rise to massive buried surfaces that are required for overcoming the repulsive force between cells, leading to bacterial cell-cell interaction and formation of complex microcolonies.

    Metadata

    Item Type: Article
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Sarah Hall
    Date Deposited: 20 Jan 2014 15:17
    Last Modified: 06 Dec 2016 10:46
    URI: http://eprints.bbk.ac.uk/id/eprint/9024

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