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    The effect of preincubation period and culture medium on early development of isolated blastoderm of Japanese quail

    Babiker, E.M. and Baggott, Glenn K. (2009) The effect of preincubation period and culture medium on early development of isolated blastoderm of Japanese quail. Journal of Genetic Engineering and Biotechnology 7 (1), pp. 29-34. ISSN 1687-157X.

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    Abstract

    Using a modification of the method of New (1955), preincubated blasoderms of Japanese quail were cultured on different culture media up to 72 h of incubation and the production of subembryonic fluid (SEF) and developmental components were investigated. Preincubation of 18 h and 48 h sustained measurable amounts of SEF and these coincided with heart pumping and development of area vasculosa. Of different culture media used, native albumen and albumen dialysed with tubing of retaining size permitting molecules of mw>2000, produced measurable amounts of SEF and showed better developmental components such as area vasculosa, length of the embryo, area pelluscida and almost showed identical pattern of radial increase of the explanted blastoderms.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): the effect, period, culture medium, early development
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Depositing User: Sarah Hall
    Date Deposited: 21 Jan 2014 09:44
    Last Modified: 21 Jan 2014 09:44
    URI: http://eprints.bbk.ac.uk/id/eprint/9076

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