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    Identification of a key residue for Oligomerisation and pore-formation of Clostridium perfringens NetB

    da Costa, S. and Savva, Christos G. and Bokori-Brown, M. and Naylor, Claire E. and Moss, David S. and Basak, Ajit K. and Titball, R. (2014) Identification of a key residue for Oligomerisation and pore-formation of Clostridium perfringens NetB. Toxins 6 (3), pp. 1049-1061. ISSN 2072-6651.

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    Abstract

    Necrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant economic damage to the poultry industry worldwide. In this study, site-directed mutagenesis was used to identify amino acids that play a role in NetB oligomerisation and pore-formation. NetB K41H showed significantly reduced toxicity towards LMH cells and human red blood cells relative to wild type toxin. NetB K41H was unable to oligomerise and form pores in liposomes. These findings suggest that NetB K41H could be developed as a genetic toxoid vaccine to protect against necrotic enteritis.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): NetB, pore-forming toxin, Clostridium perfringens, necrotic enteritis
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 18 Mar 2014 15:18
    Last Modified: 06 Dec 2016 10:43
    URI: http://eprints.bbk.ac.uk/id/eprint/9367

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