Studying antibody repertoires with next-generation sequencing

Lees, William and Shepherd, Adrian J. (2016) Studying antibody repertoires with next-generation sequencing. In: Keith, J.M. (ed.) Bioinformatics. Methods in Molecular Biology 1526. New York, U.S.: Humana Press, pp. 257-270. ISBN 9781493966110.

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Official URL: http://doi.org/10.1007/978-1-4939-6613-4_15

Abstract

Next-generation sequencing is making it possible to study the antibody repertoire of an organism in unprecedented detail, and, by so doing, to characterize its behavior in the response to infection and in pathological conditions such as autoimmunity and cancer. The polymorphic nature of the repertoire poses unique challenges that rule out the use of many commonly used NGS methods and require tradeoffs to be made when considering experimental design. We outline the main contexts in which antibody repertoire analysis has been used, and summarize the key tools that are available. The humoral immune response to vaccination has been a particular focus of repertoire analyses, and we review the key conclusions and methods used in these studies.

Metadata

Item Type:	Book Section
Additional Information:	Series ISSN: 1064-3745
Keyword(s) / Subject(s):	Antibodies, Antibodyome, Repertoire analysis, Rep-Seq, Next generation sequencing
School:	Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
Research Centres and Institutes:	Data Analytics, Birkbeck Institute for
Depositing User:	Administrator
Date Deposited:	08 Mar 2017 13:06
Last Modified:	02 Aug 2023 17:30
URI:	https://eprints.bbk.ac.uk/id/eprint/17763

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