BIROn - Birkbeck Institutional Research Online

Screening ion-channel ligand interactions with passive pumping in a microfluidic bilayer lipid membrane chip

Saha, S.C. and Powl, Andrew M. and Wallace, Bonnie A. and de Planque, M.R.R. and Morgan, H. (2015) Screening ion-channel ligand interactions with passive pumping in a microfluidic bilayer lipid membrane chip. Biomicrofluidics 9 (1), ISSN 1932-1058.

[img] Text
11496.pdf - Published Version of Record
Restricted to Repository staff only

Download (2MB) | Request a copy

Abstract

We describe a scalable artificial bilayer lipid membrane platform for rapid electrophysiological screening of ion channels and transporters. A passive pumping method is used to flow microliter volumes of ligand solution across a suspended bilayer within a microfluidic chip. Bilayers are stable at flow rates up to ∼0.5 μl/min. Phospholipid bilayers are formed across a photolithographically defined aperture made in a dry film resist within the microfluidic chip. Bilayers are stable for many days and the low shunt capacitance of the thin film support gives low-noise high-quality single ion channel recording. Dose-dependent transient blocking of α-hemolysin with β-cyclodextrin (β-CD) and polyethylene glycol is demonstrated and dose-dependent blocking studies of the KcsA potassium channel with tetraethylammonium show the potential for determining IC50 values. The assays are fast (30 min for a complete IC50 curve) and simple and require very small amounts of compounds (100 μg in 15 μl). The technology can be scaled so that multiple bilayers can be addressed, providing a screening platform for ion channels, transporters, and nanopores.

Metadata

Item Type: Article
School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
Research Centres and Institutes: Bioinformatics, Bloomsbury Centre for (Closed), Structural Molecular Biology, Institute of (ISMB)
Depositing User: Administrator
Date Deposited: 23 Jan 2015 11:36
Last Modified: 15 Jul 2025 12:36
URI: https://eprints.bbk.ac.uk/id/eprint/11496

Statistics

6 month trend
0Downloads
6 month trend
529Hits

Additional statistics are available via IRStats2.

Archive Staff Only (login required)

Edit/View Item
Edit/View Item