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    XNA ligation using T4 DNA ligase in crowding conditions

    Kestemont, D. and Renders, M. and Leonczak, P. and Abramov, M. and Schepers, G. and Pinheiro, Vitor B. and Rozenski, J. and Herdewijn, P. (2018) XNA ligation using T4 DNA ligase in crowding conditions. Chemical Communications 54 , pp. 6408-6411. ISSN 1359-7345.

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    Abstract

    T4 DNA ligase is capable of ligating 2’OMe-RNA duplexes, HNA, LNA and FANA mixed sequences in the presence of 10% w/v PEG8000 and 3 M betaine. The enzymatic joining of oligonucleotides containing multiple consecutive XNA nucleotides at the ligation site has not been reported before.

    Metadata

    Item Type: Article
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Depositing User: Dr Vitor Pinheiro
    Date Deposited: 08 Jun 2018 11:35
    Last Modified: 28 Jul 2019 21:21
    URI: http://eprints.bbk.ac.uk/id/eprint/22681

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