BIROn - Birkbeck Institutional Research Online

    Thermal melt circular dichroism spectroscopic studies for identifying stabilising amphipathic molecules for the voltage-gated sodium channel NavMs

    Ireland, Sam and Sula, Altin and Wallace, Bonnie A. (2017) Thermal melt circular dichroism spectroscopic studies for identifying stabilising amphipathic molecules for the voltage-gated sodium channel NavMs. Biopolymers , e23067. ISSN 0006-3525. (In Press)

    [img]
    Preview
    Text
    23548.pdf - Published Version of Record
    Available under License Creative Commons Attribution.

    Download (1MB) | Preview

    Abstract

    Purified integral membrane proteins require amphipathic molecules to maintain their solubility in aqueous solutions. These complexes, in turn, are used in studies to characterise the protein structures by a variety of biophysical and structural techniques, including spectroscopy, crystallography, and cryo‐electron microscopy. Typically the amphilphiles used have been detergent molecules, but more recently they have included amphipols, which are polymers of different sizes and compositions designed to create smaller, more well‐defined solubilised forms of the membrane proteins. In this study we used circular dichroism spectroscopy to compare the secondary structures and thermal stabilities of the NavMs voltage‐gated sodium channel in different amphipols and detergents as a means of identifying amphipathic environments that maximally maintain the protein structure whilst providing a stabilising environment. These types of characterisations also have potential as means of screening for sample types that may be more suitable for crystallisation and/or cryo‐electron microscopy structure determinations.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): Biophysics, Organic Chemistry, Biochemistry, Biomaterials, General Medicine
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    SWORD Depositor: Mr Joe Tenant
    Depositing User: Mr Joe Tenant
    Date Deposited: 21 Aug 2018 10:52
    Last Modified: 27 Jul 2019 18:20
    URI: http://eprints.bbk.ac.uk/id/eprint/23548

    Statistics

    Downloads
    Activity Overview
    102Downloads
    33Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item