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    Cryo-TEM and Biological Structure Determination

    Manka, Szymon and Moores, Carolyn A. (2019) Cryo-TEM and Biological Structure Determination. In: Ramesh, V. (ed.) Biomolecular and Bioanalytical Techniques: Theory, Methodology and Applications. John Wiley & Sons, Ltd, pp. 471-499. ISBN 9781119483960.

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    Abstract

    Cryo‐electron microscopy (cryo‐EM) enables unprecedented insight into the molecular machinery that drives cell life. It involves the study of frozen hydrated samples preserved in a thin layer of vitreous ice, imaged in a transmission electron microscope. The electron radiation used for imaging also damages the samples under study, posing a major limitation on the dose used. The resulting images therefore have very low contrast, requiring that many different 2D views of samples are collected and combined computationally to yield the 3D structures of interest. The field has recently undergone a revolution such that visualisation of a wide range of macromolecular complexes has dramatically improved, including structure determination at atomic resolution. Developments in hardware and software have also increased experimental throughput, making cryo‐EM a central technique in structural and cell biology. The ability of cryo‐EM to bridge biological scales – from atoms to cells – provides an excellent complement to other methods.

    Metadata

    Item Type: Book Section
    Additional Information: ** From Crossref via Jisc Publications Router ** History: epub 15-03-2019; issued 15-03-2019.
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    SWORD Depositor: Mr Joe Tenant
    Depositing User: Mr Joe Tenant
    Date Deposited: 03 May 2019 12:36
    Last Modified: 03 May 2019 12:36
    URI: http://eprints.bbk.ac.uk/id/eprint/26833

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