BIROn - Birkbeck Institutional Research Online

    Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri

    Sgro, G.G. and Costa, Tiago and Cenens, W. and Souza, D.P. and Cassago, A. and Coutinho de Oliveira, L. and Salinas, R.K. and Portugal, R.V. and Farah, C.S. and Waksman, Gabriel (2018) Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri. Nature Microbiology 3 (12), pp. 1429-1440. ISSN 2058-5276.

    [img]
    Preview
    Text
    26923.pdf - Author's Accepted Manuscript

    Download (217MB) | Preview

    Abstract

    Type IV secretion (T4S) systems form the most common and versatile class of secretion systems in bacteria, capable of injecting both proteins and DNAs into host cells. T4S systems are typically composed of 12 components that form 2 major assemblies: the inner membrane complex embedded in the inner membrane and the core complex embedded in both the inner and outer membranes. Here we present the 3.3 Å-resolution cryo-electron microscopy model of the T4S system core complex from Xanthomonas citri, a phytopathogen that utilizes this system to kill bacterial competitors. An extensive mutational investigation was performed to probe the vast network of protein–protein interactions in this 1.13-MDa assembly. This structure expands our knowledge of the molecular details of T4S system organization, assembly and evolution.

    Metadata

    Item Type: Article
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Depositing User: Administrator
    Date Deposited: 27 Mar 2019 14:42
    Last Modified: 26 Jul 2019 21:20
    URI: http://eprints.bbk.ac.uk/id/eprint/26923

    Statistics

    Downloads
    Activity Overview
    6Downloads
    34Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item