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    Cyclic AMP signalling controls key components of malaria parasite host cell invasion machinery

    Patel, A. and Perrin, A.J. and Flynn, H.R. and Bisson, Claudine and Withers-Martinez, C. and Treeck, M. and Flueck, C. and Nicastro, G. and Martin, S.R. and Ramos, A. and Gilberger, T.W. and Snijders, A.P. and Blackman, M.J. and Baker, D.A. (2019) Cyclic AMP signalling controls key components of malaria parasite host cell invasion machinery. PLOS Biology 17 (5), e3000264. ISSN 1545-7885.

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    Abstract

    Cyclic AMP (cAMP) is an important signalling molecule across evolution, but its role in malaria parasites is poorly understood. We have investigated the role of cAMP in asexual blood stage development of Plasmodium falciparum through conditional disruption of adenylyl cyclase beta (ACβ) and its downstream effector, cAMP-dependent protein kinase (PKA). We show that both production of cAMP and activity of PKA are critical for erythrocyte invasion, whilst key developmental steps that precede invasion still take place in the absence of cAMP-dependent signalling. We also show that another parasite protein with putative cyclic nucleotide binding sites, Plasmodium falciparum EPAC (PfEpac), does not play an essential role in blood stages. We identify and quantify numerous sites, phosphorylation of which is dependent on cAMP signalling, and we provide mechanistic insight as to how cAMP-dependent phosphorylation of the cytoplasmic domain of the essential invasion adhesin apical membrane antigen 1 (AMA1) regulates erythrocyte invasion.

    Metadata

    Item Type: Article
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    SWORD Depositor: Mr Joe Tenant
    Depositing User: Mr Joe Tenant
    Date Deposited: 28 Jun 2019 08:24
    Last Modified: 29 Jul 2019 01:46
    URI: http://eprints.bbk.ac.uk/id/eprint/27781

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