Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis

Ryan, C.P. and Smith, M.E.B. and Schumacher, F.F. and Grohmann, D. and Papaioannou, D. and Waksman, Gabriel and Werner, F. and Baker, J.R. and Caddick, S. (2011) Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis. Chemical Communications 47 (19), pp. 5452-5454. ISSN 1359-7345.

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Official URL: http://dx.doi.org/10.1039/C1CC11114K

Abstract

Controlling maleimide hydrolysis allows the modular construction of bromomaleimide-mediated bioconjugates which are either stable or cleavable in an aqueous, thiol-mediated reducing environment. The application of this methodology to reversible protein biotinylation, the irreversible labeling of peptide disulfide bonds and the assembly of stable, fluorescein-labelled glycoprotein mimics is described.

Metadata

Item Type:	Article
School:	School of Science > Biological Sciences
Research Centres and Institutes:	Structural Molecular Biology, Institute of (ISMB)
Depositing User:	Administrator
Date Deposited:	20 Jun 2011 09:32
Last Modified:	06 Dec 2016 10:46
URI:	http://eprints.bbk.ac.uk/id/eprint/3708

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