BIROn - Birkbeck Institutional Research Online

    The structure of Herpesvirus Fusion Glycoprotein B-Bilayer Complex reveals the protein-membrane and lateral protein-protein interaction

    Maurer, U.E. and Zeev-Ben-Mordehai, T. and Pandurangan, Arun and Cairns, T.M. and Hannah, B.P. and Whitbeck, J.C. and Eisenberg, R.J. and Cohen, G.H. and Topf, Maya and Huiskonen, J.T. and Grünewald, K. (2013) The structure of Herpesvirus Fusion Glycoprotein B-Bilayer Complex reveals the protein-membrane and lateral protein-protein interaction. Structure 21 (8), pp. 1396-1405. ISSN 0969-2126.

    [img]
    Preview
    Text
    7994.pdf - Published Version of Record
    Available under License Creative Commons Attribution.

    Download (3MB) | Preview

    Abstract

    Glycoprotein B (gB) is a key component of the complex herpesvirus fusion machinery. We studied membrane interaction of two gB ectodomain forms and present an electron cryotomography structure of the gB-bilayer complex. The two forms differed in presence or absence of the membrane proximal region (MPR) but showed an overall similar trimeric shape. The presence of the MPR impeded interaction with liposomes. In contrast, the MPR-lacking form interacted efficiently with liposomes. Lateral interaction resulted in coat formation on the membranes. The structure revealed that interaction of gB with membranes was mediated by the fusion loops and limited to the outer membrane leaflet. The observed intrinsic propensity of gB to cluster on membranes indicates an additional role of gB in driving the fusion process forward beyond the transient fusion pore opening and subsequently leading to fusion pore expansion.

    Metadata

    Item Type: Article
    School: Birkbeck Schools and Departments > School of Science > Psychological Sciences
    Research Centres and Institutes: Bioinformatics, Bloomsbury Centre for (Closed), Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 12 Aug 2013 11:41
    Last Modified: 30 Jun 2020 14:44
    URI: http://eprints.bbk.ac.uk/id/eprint/7994

    Statistics

    Downloads
    Activity Overview
    985Downloads
    157Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item