BIROn - Birkbeck Institutional Research Online

    Characterisation of a putative AraC transcriptional regulator from Mycobacterium smegmatis

    Evangelopoulos, Dimitrios and Gupta, Antima and Lack, N. and Maitra, Arundhati and ten Bokum, A.M.C. and Kendall, Sharon L. and Sim, E. and Bhakta, Sanjib (2014) Characterisation of a putative AraC transcriptional regulator from Mycobacterium smegmatis. Tuberculosis 94 (6), pp. 664-671. ISSN 1472-9792.

    10911.pdf - Published Version of Record
    Available under License Creative Commons Attribution.

    Download (1MB) | Preview


    MSMEG_0307 is annotated as a transcriptional regulator belonging to the AraC protein family and is located adjacent to the arylamine N-acetyltransferase (nat) gene in Mycobacterium smegmatis, in a gene cluster, conserved in most environmental mycobacterial species. In order to elucidate the function of the AraC protein from the nat operon in M. smegmatis, two conserved palindromic DNA motifs were identified using bioinformatics and tested for protein binding using electrophoretic mobility shift assays with a recombinant form of the AraC protein. We identified the formation of a DNA:AraC protein complex with one of the motifs as well as the presence of this motif in 20 loci across the whole genome of M. smegmatis, supporting the existence of an AraC controlled regulon. To characterise the effects of AraC in the regulation of the nat operon genes, as well as to gain further insight into its function, we generated a ΔaraC mutant strain where the araC gene was replaced by a hygromycin resistance marker. The level of expression of the nat and MSMEG_0308 genes was down-regulated in the ΔaraC strain when compared to the wild type strain indicating an activator effect of the AraC protein on the expression of the nat operon genes.


    Item Type: Article
    Keyword(s) / Subject(s): AraC, Transcriptional regulator, Mycobacteria, Mycobacterial two-hybrid system, nat operon, Protein–protein interaction
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Research Centres and Institutes: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 05 Nov 2014 12:29
    Last Modified: 02 Aug 2023 17:13


    Activity Overview
    6 month trend
    6 month trend

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item