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    Ultra high-performance liquid chromatography of porphyrins

    Benton, C.M. and Lim, Chang-Kee and Ritchie, H.J. and Moniz, C. and Jones, D.J.L. (2011) Ultra high-performance liquid chromatography of porphyrins. Biomedical Chromatography 26 (3), pp. 331-337. ISSN 0269-3879.

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    An ultra high-performance liquid chromatographic (UHPLC) system was developed and optimized for the separation of porphyrins of clinical interest. Optimum conditions for the simultaneous separation of uroporphyrin, hepta-, hexa-, penta-carboxylic acid porphyrins and coproporphyrin and their type I and III isomers on a Thermo Hypersil BDS C18 column (2.4 µm particle size, 100 × 2.1 mm i.d.) using a gradient elution with 10% (v/v) acetonitrile in 1.0 m ammonium acetate buffer (pH 5.16) and 10% (v/v) acetonitrile in methanol at a flow-rate of 0.4 mL/min. The effect of mobile phase buffer molarity on the sensitivity of fluorescence detection and resolution of porphyrin isomers was investigated. The method was successfully applied to the analysis of porphyrins extracted from the urine and faeces of patients with various human porphyrias. Copyright © 2011 John Wiley & Sons, Ltd.


    Item Type: Article
    Keyword(s) / Subject(s): UHPLC, porphyrins, porphyrias, porphyrin profiles in urine and faeces
    School: School of Science > Biological Sciences
    Depositing User: Administrator
    Date Deposited: 02 Dec 2014 09:44
    Last Modified: 02 Dec 2014 09:44


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