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    Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR

    Kaplan, M. and Cukkemane, A. and van Zundert, G.C.P and Narasimhan, S. and Daniëls, M. and Mance, D. and Waksman, Gabriel and Bonvin, A.M.J.J and Fronzes, R. and Folkers, G.E. and Baldus, M. (2015) Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR. Nature Methods 12 (7), pp. 649-652. ISSN 1548-7091.

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    Abstract

    Studying biomolecules at atomic resolution in their native environment is the ultimate aim of structural biology. We investigated the bacterial type IV secretion system core complex (T4SScc) by cellular dynamic nuclear polarization–based solid-state nuclear magnetic resonance spectroscopy to validate a structural model previously generated by combining in vitro and in silico data. Our results indicate that T4SScc is well folded in the cellular setting, revealing protein regions that had been elusive when studied in vitro.

    Metadata

    Item Type: Article
    School: School of Science > Biological Sciences
    Research Centres and Institutes: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 22 May 2015 09:27
    Last Modified: 06 Dec 2016 10:45
    URI: https://eprints.bbk.ac.uk/id/eprint/12128

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