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    Characterization of the Prokaryotic Sodium Channel NavSp Pore with a Microfluidic Bilayer Platform

    Zhang, Z. and Saha, S.C. and Henderson, Alexander J. and Powl, Andrew M. and Wallace, Bonnie A. and de Planque, M.R.R. and Morgan, H. (2015) Characterization of the Prokaryotic Sodium Channel NavSp Pore with a Microfluidic Bilayer Platform. PLOS ONE 10 (7), e0131286. ISSN 1932-6203.

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    Abstract

    This paper describes the use of a newly-developed micro-chip bilayer platform to examine the electrophysiological properties of the prokaryotic voltage-gated sodium channel pore (NavSp) from Silicibacter pomeroyi. The platform allows up to 6 bilayers to be analysed simultaneously. Proteoliposomes were incorporated into suspended lipid bilayers formed within the microfluidic bilayer chips. The chips provide access to bilayers from either side, enabling the fast and controlled titration of compounds. Dose-dependent modulation of the opening probability by the channel blocking drug nifedipine was measured and its IC50 determined.

    Metadata

    Item Type: Article
    School: School of Science > Biological Sciences
    Research Centres and Institutes: Bioinformatics, Bloomsbury Centre for (Closed), Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 07 Jul 2015 13:01
    Last Modified: 10 Jun 2021 18:13
    URI: https://eprints.bbk.ac.uk/id/eprint/12503

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