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    Modulation of reactivity in the cavity of liposomes promotes the formation of peptide bonds

    Grochmal, Anna and Prout, Luba and Makin-Taylor, Robert and Prohens, R. and Tomas, Salvador (2015) Modulation of reactivity in the cavity of liposomes promotes the formation of peptide bonds. Journal of the American Chemical Society 137 (38), pp. 12269-12275. ISSN 0002-7863.

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    In living cells reactions take place in membrane bound compartments, often in response to changes in the environment. Learning how the reactions are influenced by this compartmentalization will help us gain an optimal understanding of living organisms at the molecular level and, at the same time, will offer vital clues on the behavior of simple compartmentalized systems, such as prebiotic precursors of cells and cell-inspired artificial systems. In this work we show that a reactive building block (an activated amino acid derivative) trapped in the cavity of a liposome is protected against hydrolysis and reacts nearly quantitatively with another building block, which is membrane-permeable and free in solution, to form the dipeptide. By contrast, when found outside the liposome, the hydrolysis of the activated amino acid is the prevalent reaction, showing that the cavity of the liposomes promotes the formation of peptide bonds. We attribute this result to the large lipid concentration in small compartments from the point of view of a membrane-impermeable molecule. Based on this result we show how the outcome of the reaction can be predicted as a function of the size of the compartment. The implications of these results on the behavior of biomolecules in cell compartments, abiogenesis and the design of artificial cell-inspired systems are considered


    Item Type: Article
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Research Centres and Institutes: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 21 Sep 2015 13:37
    Last Modified: 02 Aug 2023 17:18


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