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    γ-TEMPy: simultaneous fitting of components in 3D-EM maps of their assembly using a genetic algorithm

    Pandurangan, Arun and Vasishtan, D. and Alber, F. and Topf, Maya (2015) γ-TEMPy: simultaneous fitting of components in 3D-EM maps of their assembly using a genetic algorithm. Structure 23 (12), pp. 2365-2376. ISSN 0969-2126.

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    We have developed a genetic algorithm for building macromolecular complexes using only a 3D-electron microscopy density map and the atomic structures of the relevant components. For efficient sampling the method uses map feature points calculated by vector quantisation. The fitness function combines a mutual information score that quantifies the goodness-of-fit with a penalty score that helps to avoid clashes between components. Testing the method on ten assemblies (containing 3 to 8 protein components) and simulated density maps at 10, 15, and 20 Å resolution resulted in identification of the correct topology in 90%, 70% and 60% of the cases, respectively. We further tested it on four assemblies with experimental maps at 7.2-23.5 Å resolution, showing the ability of the method to identify the correct topology in all cases. We have also demonstrated the importance of the map feature-point quality on assembly fitting in the lack of additional experimental information.


    Item Type: Article
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Research Centres and Institutes: Bioinformatics, Bloomsbury Centre for (Closed), Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 24 Nov 2015 11:29
    Last Modified: 02 Aug 2023 17:19


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