Jeremy, G. and Stevens, J. and Lowe, Alan R. (2016) Single-molecule imaging to characterise the transport mechanism of the Nuclear Pore Complex. In: Leake, M.C. (ed.) Chromosome Architecture: Methods and Protocols. Methods in Molecular Biology 1431. New York, U.S.: Springer New York, pp. 17-35. ISBN 9781493936298.
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Abstract
In the eukaryotic cell, a large macromolecular channel, known as the Nuclear Pore Complex (NPC), mediates all molecular transport between the nucleus and cytoplasm. In recent years, single-molecule fluorescence (SMF) imaging has emerged as a powerful tool to study the molecular mechanism of transport through the NPC. More recently, techniques such as Single-Molecule Localisation Microscopy (SMLM) have enabled the spatial and temporal distribution of cargos, transport receptors and even structural components of the NPC to be determined with nanometre accuracy. In this protocol, we describe a method to study the position and/or motion of individual molecules transiting through the NPC with high spatial and temporal precision.
Metadata
| Item Type: | Book Section |
|---|---|
| Additional Information: | Series ISSN: 1064-3745: The original publication is available at www.springerlink.com |
| Keyword(s) / Subject(s): | Nucleus, Nuclear Pore Complex, Single-Molecule Tracking, Super-resolution Microscopy |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Research Centres and Institutes: | Structural Molecular Biology, Institute of (ISMB) |
| Depositing User: | Administrator |
| Date Deposited: | 07 Jul 2016 14:00 |
| Last Modified: | 08 Aug 2025 13:57 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/14935 |
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