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    Arylamine N-Acetyltransferase in Mycobacteria

    Evangelopoulos, Dimitrios and Bhakta, Sanjib (2018) Arylamine N-Acetyltransferase in Mycobacteria. In: Laurieri, N. and Sim, E. (eds.) Arylamine N-acetyltransferases in Health and Disease: From Pharmacogenetics to Drug Discovery and Diagnostics. World Scientific, pp. 303-324. ISBN 9789813232006.

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    Abstract

    Arylamine N-acetyltransferase (NAT) was first identified in humans for its role in metabolising the anti-tubercular drug isoniazid. Later functional NAT proteins were identified in bacteria including Mycobacterium tuberculosis where they were found to N-acetylate a range of arylamines, hydrazines and hydralazines both in vitro and in vivo. From gene deletion and genomic studies in mycobacteria, it became clear that NAT in Mycobacterium bovis Bacillus Calmette–Guérin (BCG) had an endogenous role in cell-wall metabolism, cholesterol degradation and the nat gene was part of an essential gene cluster for intra-cellular survival of the pathogen M. tuberculosis; this has paved the way for validation of NAT and other proteins encoded in the gene cluster as novel therapeutic targets for anti-tubercular drug design and discovery. This chapter reviews the genomic organisation of the nat gene and the function of the NAT protein in mycobacteria, including the location of the nat gene in the genome. The role of nat and its neighbouring genes, their endogenous substrates and the regulation of their expression in fast-growing environmental and slow-growing pathogenic intra-cellular mycobacteria is also covered.

    Metadata

    Item Type: Book Section
    Keyword(s) / Subject(s): Mycobacterium, tuberculosis (TB), antimicrobial resistance (AMR), arylamine N-acetyltransferase (NAT), gene cluster/operon analysis, antitubercular drug discovery, SPOTi (whole-cell phenotypic assay), lipid/cholesterol metabolism, environmental mycobacteria, intra-cellular pathogens
    School: School of Science > Biological Sciences
    SWORD Depositor: Mr Joe Tenant
    Depositing User: Mr Joe Tenant
    Date Deposited: 23 Apr 2018 12:57
    Last Modified: 28 Jul 2020 09:43
    URI: https://eprints.bbk.ac.uk/id/eprint/22058

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