BIROn - Birkbeck Institutional Research Online

    A structurally conserved motif in γ-herpesvirus uracil-DNA glycosylases elicits duplex nucleotide-flipping

    Earl, Chris and Bagneris, Claire and Zeman, K. and Cole, Ambrose and Barrett, Tracey and Savva, Renos (2018) A structurally conserved motif in γ-herpesvirus uracil-DNA glycosylases elicits duplex nucleotide-flipping. Nucleic Acids Research 46 (8), pp. 4286-4300. ISSN 0305-1048.

    [img]
    Preview
    Text
    gky217.pdf - Published Version of Record
    Available under License Creative Commons Attribution.

    Download (8MB) | Preview

    Abstract

    Efficient γ-herpesvirus lytic phase replication requires a virally encoded UNG-type uracil-DNA glycosylase as a structural element of the viral replisome. Uniquely, γ-herpesvirus UNGs carry a seven or eight residue insertion of variable sequence in the otherwise highly conserved minor-groove DNA binding loop. In Epstein–Barr Virus [HHV-4] UNG, this motif forms a disc-shaped loop structure of unclear significance. To ascertain the biological role of the loop insertion, we determined the crystal structure of Kaposi’s sarcoma-associated herpesvirus [HHV-8] UNG (kUNG) in its product complex with a uracil-containing dsDNA, as well as two structures of kUNG in its apo state. We find the disc-like conformation is conserved, but only when the kUNG DNA-binding cleft is occupied. Surprisingly, kUNG uses this structure to flip the orphaned partner base of the substrate deoxyuridine out of the DNA duplex while retaining canonical UNG deoxyuridine-flipping and catalysis. The orphan base is stably posed in the DNA major groove which, due to DNA backbone manipulation by kUNG, is more open than in other UNG–dsDNA structures. Mutagenesis suggests a model in which the kUNG loop is pinned outside the DNA-binding cleft until DNA docking promotes rigid structuring of the loop and duplex nucleotide flipping, a novel observation for UNGs.

    Metadata

    Item Type: Article
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Research Centres and Institutes: Structural Molecular Biology, Institute of (ISMB), Innovation Management Research, Birkbeck Centre for
    Depositing User: Tracey Barrett
    Date Deposited: 11 Apr 2018 17:28
    Last Modified: 02 Aug 2023 17:41
    URI: https://eprints.bbk.ac.uk/id/eprint/22097

    Statistics

    Activity Overview
    6 month trend
    288Downloads
    6 month trend
    261Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item