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    Terminal regions confer plasticity to the tetrameric assembly of human HspB2 and HspB3

    Clark, Alice and Egberts, W.V. and Kondrat, F.D.L. and Hilton, G.R. and Ray, N.J. and Cole, Ambrose and Carver, J.A. and Benesch, J.L.P. and Keep, Nicholas H. and Boelens, W.C. and Slingsby, Christine (2018) Terminal regions confer plasticity to the tetrameric assembly of human HspB2 and HspB3. Journal of Molecular Biology 430 (18B), pp. 3297-3310. ISSN 0022-2836.

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    Heterogeneity in small heat shock proteins (sHsps) spans multiple spatiotemporal regimes – from fast fluctuations of part of the protein, to conformational variability of tertiary structure, plasticity of the interfaces, and polydispersity of the inter-converting, and co-assembling oligomers. This heterogeneity and dynamic nature of sHsps has significantly hindered their structural characterisation. Atomic-coordinates are particularly lacking for vertebrate sHsps, where most available structures are of extensively truncated homomers. sHsps play important roles in maintaining protein levels in the cell and therefore in organismal health and disease. HspB2 and HspB3 are vertebrate sHsps that are found co-assembled in neuromuscular cells, and variants thereof are associated with disease. Here, we present the structure of human HspB2/B3, which crystallised as a hetero-tetramer in a 3:1 ratio. In the HspB2/B3 tetramer, the four a-crystallin domains (ACDs) assemble into a flattened tetrahedron which is pierced by two non-intersecting approximate dyads. Assembly is mediated by flexible “nuts and bolts” involving IXI/V motifs from terminal regions filling ACD pockets. Parts of the N-terminal region bind in an unfolded conformation into the anti-parallel shared ACD dimer grooves. Tracts of the terminal regions are not resolved, most likely due to their disorder in the crystal lattice. This first structure of a full-length human sHsp heteromer reveals the heterogeneous interactions of the terminal regions and suggests a plasticity that is important for the cytoprotective functions of sHsps.


    Item Type: Article
    Keyword(s) / Subject(s): a-crystallin domain, asymmetric heteromer, heat shock protein, molecular chaperone, polydispersity
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Research Centres and Institutes: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Nick Keep
    Date Deposited: 27 Jun 2018 13:32
    Last Modified: 02 Aug 2023 17:42


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