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    Insights into Centromere DNA bending revealed by the Cryo-EM structure of the core Centromere Binding Factor 3 with Ndc10

    Zhang, W. and Lukoynova, Natalia and Miah, S. and Lucas, J. and Vaughan, Cara K. (2018) Insights into Centromere DNA bending revealed by the Cryo-EM structure of the core Centromere Binding Factor 3 with Ndc10. Cell Reports 24 (3), pp. 744-754. ISSN 2211-1247.

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    Abstract

    The Centromere Binding Factor 3 (CBF3) complex binds the third Centromere DNA Element in organisms with point centromeres, such as S. cerevisiae. It is an essential complex for assembly of the kinetochore in these organisms as it facilitates genetic centromere specification and allows association of all other kinetochore components. We determined high resolution structures of the core complex of CBF3 alone and in association with a monomeric construct of Ndc10, using cryo-EM. We identify the DNA binding site of the complex and present a model in which CBF3 induces a tight bend in centromeric DNA, thus facilitating assembly of the centromeric nucleosome.

    Metadata

    Item Type: Article
    School: School of Science > Biological Sciences
    Depositing User: Cara Vaughan
    Date Deposited: 09 Aug 2018 12:48
    Last Modified: 22 Feb 2021 13:53
    URI: https://eprints.bbk.ac.uk/id/eprint/23471

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