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    Invasive and adherent bacterial pathogens co-opt host clathrin for infection

    Veiga, E. and Guttman, J.A. and Bonazzi, M. and Boucrot, Emmanuel and Toledo-Arana, A. and Lin, A.E. and Cossart, P. and Enninga, J. and Pizarro-Cerdá, J. and Finlay, B. and Kirchhausen, T. and Cossart, P. (2007) Invasive and adherent bacterial pathogens co-opt host clathrin for infection. Cell Host & Microbe 2 (5), pp. 340-351. ISSN 1931-3128.

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    Infection by the bacterium Listeria monocytogenes depends on host cell clathrin. To determine whether this requirement is widespread, we analyzed infection models using diverse bacteria. We demonstrated that bacteria that enter cells following binding to cellular receptors (termed “zippering” bacteria) invade in a clathrin-dependent manner. In contrast, bacteria that inject effector proteins into host cells in order to gain entry (termed “triggering” bacteria) invade in a clathrin-independent manner. Strikingly, enteropathogenic Escherichia coli (EPEC) required clathrin to form actin-rich pedestals in host cells beneath adhering bacteria, even though this pathogen remains extracellular. Furthermore, clathrin accumulation preceded the actin rearrangements necessary for Listeria entry. These data provide evidence for a clathrin-based entry pathway allowing internalization of large objects (bacteria and ligand-coated beads) and used by “zippering” bacteria as part of a general mechanism to invade host mammalian cells. We also revealed a nonendocytic role for clathrin required for extracellular EPEC infections.


    Item Type: Article
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Depositing User: Sarah Hall
    Date Deposited: 30 Apr 2019 15:37
    Last Modified: 02 Aug 2023 17:51


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