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    Salmonella maintains the integrity of its intracellular vacuole through the action of SifA

    Carmen, R. and Beuzón, S.M. and Unsworth, K.E. and Ruíz‐Albert, J. and Garvis, S. and Waterman, S.R. and Ryder, T.A. and Boucrot, Emmanuel and Holden, D.W. (2000) Salmonella maintains the integrity of its intracellular vacuole through the action of SifA. Embo Journal 19 (13), pp. 3235-3249. ISSN 0261-4189.

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    A method based on the Competitive Index was used to identify Salmonella typhimurium virulence gene interactions during systemic infections of mice. Analysis of mixed infections involving single and double mutant strains showed that OmpR, the type III secretion system of Salmonella pathogenicity island 2 (SPI‐2) and SifA [required for the formation in epithelial cells of lysosomal glycoprotein (lgp)‐containing structures, termed Sifs] are all involved in the same virulence function. sifA gene expression was induced after Salmonella entry into host cells and was dependent on the SPI‐2 regulator ssrA. A sifA− mutant strain had a replication defect in macrophages, similar to that of SPI‐2 and ompR− mutant strains. Whereas wild‐type and SPI‐2 mutant strains reside in vacuoles that progressively acquire lgps and the vacuolar ATPase, the majority of sifA− bacteria lost their vacuolar membrane and were released into the host cell cytosol. We propose that the wild‐type strain, through the action of SPI‐2 effectors (including SpiC), diverts the Salmonella‐containing vacuole from the endocytic pathway, and subsequent recruitment and maintenance of vacuolar ATPase/lgp‐containing membranes that enclose replicating bacteria is mediated by translocation of SifA.


    Item Type: Article
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Depositing User: Sarah Hall
    Date Deposited: 07 May 2019 12:59
    Last Modified: 02 Aug 2023 17:51


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