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    Cryo‐EM targets in CASP13: overview and evaluation of results

    Kryshtafovych, A. and Malhotra, Sony and Monastyrskyy, B. and Cragnolini, T. and Joseph, A.‐P. and Chiu, W. and Topf, Maya (2019) Cryo‐EM targets in CASP13: overview and evaluation of results. Proteins: Structure, Function, and Bioinformatics 87 (12), pp. 1128-1140. ISSN 0887-3585.

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    Structures of seven CASP13 targets were determined using cryo‐electron microscopy (cryo‐EM) technique with resolution between 3.0 and 4.0 å. We provide an overview of the experimentally derived structures and describe results of the numerical evaluation of the submitted models. The evaluation is carried out by comparing coordinates of models to those of reference structures (CASP‐style evaluation), as well as checking goodness‐of‐fit of modeled structures to the cryo‐EM density maps. The performance of contributing research groups in the CASP‐style evaluation is measured in terms of backbone accuracy, all‐atom local geometry and similarity of inter‐subunit interfaces. The results on the cryo‐EM targets are compared with those on the whole set of eighty CASP13 targets. A‐posteriori refinement of the best models in their corresponding cryo‐EM density maps resulted in structures that are very close to the reference structure, including some regions with better fit to the density.


    Item Type: Article
    Additional Information: This is the peer reviewed version of the article, which has been published in final form at the link above. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Depositing User: Administrator
    Date Deposited: 07 Oct 2019 15:33
    Last Modified: 02 Aug 2023 17:54


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