BIROn - Birkbeck Institutional Research Online

    Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters

    Dergai, O. and Cousin, P. and Gouge, Jerome and Satia, K. and Praz, V. and Kuhlman, T. and Lhôte, P. and Vannini, A. and Hernandez, N. (2018) Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters. Genes & Development 32 (9-10), pp. 711-722. ISSN 1549-5477.

    [img]
    Preview
    Text
    Dergai et al. - 2018 - Mechanism of selective recruitment of RNA polymera.pdf - Published Version of Record
    Available under License Creative Commons Attribution.

    Download (2MB) | Preview

    Abstract

    RNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and "proximal sequence element" (PSE), which recruits the SNAP complex (SNAPc). The main distinguishing feature is the presence, in the type 3 promoters only, of a TATA box, which determines Pol III specificity. To understand the mechanism by which the absence or presence of a TATA box results in specific Pol recruitment, we examined how SNAPc and general transcription factors required for Pol II or Pol III transcription of SNAPc-dependent genes (i.e., TATA-box-binding protein [TBP], TFIIB, and TFIIA for Pol II transcription and TBP and BRF2 for Pol III transcription) assemble to ensure specific Pol recruitment. TFIIB and BRF2 could each, in a mutually exclusive fashion, be recruited to SNAPc. In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.

    Metadata

    Item Type: Article
    School: School of Science > Biological Sciences
    Research Centres and Institutes: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Jerome Gouge
    Date Deposited: 03 Mar 2020 15:49
    Last Modified: 09 Jun 2021 20:06
    URI: https://eprints.bbk.ac.uk/id/eprint/31005

    Statistics

    Downloads
    Activity Overview
    67Downloads
    60Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item