BIROn - Birkbeck Institutional Research Online

    Structural basis of ligand specificity in the human pentraxins, C-reactive protein and serum amyloid P component

    Mikolajek, H. and Kolstoe, S.E. and Pye, V.E. and Mangione, P. and Pepys, M.B. and Wood, S.P. (2011) Structural basis of ligand specificity in the human pentraxins, C-reactive protein and serum amyloid P component. Journal of Molecular Recognition 24 (2), pp. 371-377. ISSN 1099-1352.

    Full text not available from this repository.

    Abstract

    The normal physiological roles of the phylogenetically conserved human plasma proteins C-reactive protein (CRP) and serum amyloid P component (SAP) are not known. Novel drugs targeting their ligand specificities are in clinical development as both proteins have significant pathophysiological effects, SAP in promoting amyloidosis and CRP in exacerbating ischemic injury. Both proteins bind to phosphoethanolamine and we show here that, under physiological conditions, phosphoethanolamine is bound with higher affinity by human SAP than by human CRP. An explanation is provided by X-ray crystal structures that show SAP residue Tyr74 allowing additional hydrophobic protein-ligand interactions compared with the equivalent Thr76 of CRP. Docking simulations show many more low energy positions for phosphoethanolamine bound by CRP than by SAP and are consistent with the crystallographic and functional binding results. These fundamental observations on structure-activity relationships will aid the design of improved pentraxin targeting drugs. Copyright (C) 2011 John Wiley & Sons, Ltd.

    Metadata

    Item Type: Article
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Depositing User: Administrator
    Date Deposited: 21 Jun 2011 09:15
    Last Modified: 02 Aug 2023 16:55
    URI: https://eprints.bbk.ac.uk/id/eprint/3675

    Statistics

    Activity Overview
    6 month trend
    0Downloads
    6 month trend
    178Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item