Cryo-EM structure of a microtubule-bound parasite kinesin motor and implications for its mechanism and inhibition
Cook, Alex and Roberts, Anthony J. and Atherton, Joe and Tewari, R. and Topf, Maya and Moores, Carolyn A. (2021) Cryo-EM structure of a microtubule-bound parasite kinesin motor and implications for its mechanism and inhibition. Journal of Biological Chemistry 297 (5), p. 101063. ISSN 0021-9258.
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Abstract
Plasmodium parasites cause malaria and are responsible annually for hundreds of thousands of deaths. Kinesins are a superfamily of microtubule-dependent ATPases that play important roles in the parasite replicative machinery, which is a potential target for anti-parasite drugs. Kinesin-5, a molecular motor that crosslinks microtubules, is an established anti-mitotic targets in other disease contexts, but its mechanism in P. falciparum is unclear. Here, we characterised P. falciparum kinesin-5 (PfK5) using cryo-EM to determine the motor’s nucleotide-dependent microtubule-bound structure, and introduced 3D classification of individual motors into our microtubule image processing pipeline to maximise our structural insights. Despite sequence divergence in PfK5, the motor exhibits classical kinesin mechanochemistry, including ATP-induced subdomain rearrangement and cover neck bundle formation, consistent with its plus-ended directed motility. We also observed that an insertion in loop5 of the PfK5 motor domain creates a different environment in the well characterised human kinesin-5 drug-binding site. Our data reveal the possibility for selective inhibition of PfK5 and can be used to inform future exploration of Plasmodium kinesins as anti-parasite targets.
Metadata
Item Type: | Article |
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Keyword(s) / Subject(s): | ATPase, anti-parasitic, cell division, cryo-EM, kinesin, malaria, mechanochemistry, microtubule, motor, Plasmodium falciparum |
School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
Depositing User: | Administrator |
Date Deposited: | 10 Aug 2021 11:16 |
Last Modified: | 02 Aug 2023 18:11 |
URI: | https://eprints.bbk.ac.uk/id/eprint/45404 |
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