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    Structural basis of substrate progression through the bacterial chaperonin cycle

    Gardner, S. and Darrow, M. and Lukoyanova, N. and Thalassinos, K. and Saibil, Helen (2023) Structural basis of substrate progression through the bacterial chaperonin cycle. Proceedings of the National Academy of Sciences of the United States of America 120 (50), e2308933120. ISSN 0027-8424.

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    Abstract

    The bacterial chaperonin GroEL-GroES promotes protein folding through ATP-regulated cycles of substrate protein binding, encapsulation, and release. Here, we have used cryoEM to determine structures of GroEL, GroEL-ADP·BeF3, and GroEL-ADP·AlF3-GroES all complexed with the model substrate Rubisco. Our structures provide a series of snapshots that show how the conformation and interactions of non-native Rubisco change as it proceeds through the GroEL-GroES reaction cycle. We observe specific charged and hydrophobic GroEL residues forming strong initial contacts with non-native Rubisco. Binding of ATP or ADP·BeF3 to GroEL-Rubisco results in the formation of an intermediate GroEL complex displaying striking asymmetry in the ATP/ADP·BeF3-bound ring. In this ring, four GroEL subunits bind Rubisco and the other three are in the GroES-accepting conformation, suggesting how GroEL can recruit GroES without releasing bound substrate. Our cryoEM structures of stalled GroEL-ADP·AlF3-Rubisco-GroES complexes show Rubisco folding intermediates interacting with GroEL-GroES via different sets of residues.

    Metadata

    Item Type: Article
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Depositing User: Helen Saibil
    Date Deposited: 09 Jan 2024 12:53
    Last Modified: 09 Jan 2024 14:26
    URI: https://eprints.bbk.ac.uk/id/eprint/52686

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