BIROn - Birkbeck Institutional Research Online

    Resolving species boundaries in a recent radiation with the Angiosperms353 probe set: the Lomatium packardiae/L. anomalum clade of the L. triternatum (Apiaceae) complex

    Ottenlips, M.V. and Mansfield, D.H. and Buerki, S. and Feist, M.A.E. and Downie, S.R. and Dodsworth, Steven and Forest, F. and Plunkett, G.M. and Smith, J.F. (2021) Resolving species boundaries in a recent radiation with the Angiosperms353 probe set: the Lomatium packardiae/L. anomalum clade of the L. triternatum (Apiaceae) complex. American Journal of Botany 108 (7), pp. 1217-1233. ISSN 0002-9122.

    [img]
    Preview
    Text
    American J of Botany - 2021 - Ottenlips - Resolving species boundaries in a recent radiation with the Angiosperms353 probe.pdf - Published Version of Record
    Available under License Creative Commons Attribution.

    Download (1MB) | Preview

    Abstract

    Premise: Speciation not associated with morphological shifts is challenging to detect unless molecular data are employed. Using Sanger-sequencing approaches, the Lomatium packardiae/L. anomalum subcomplex within the larger Lomatium triternatum complex could not be resolved. Therefore, we attempt to resolve these boundaries here. Methods: The Angiosperms353 probe set was employed to resolve the ambiguity within Lomatium triternatum species complex using 48 accessions assigned to L. packardiae, L. anomalum, or L. triternatum. In addition to exon data, 54 nuclear introns were extracted and were complete for all samples. Three approaches were used to estimate evolutionary relationships and define species boundaries: STACEY, a Bayesian coalescent-based species tree analysis that takes incomplete lineage sorting into account; ASTRAL-III, another coalescent-based species tree analysis; and a concatenated approach using MrBayes. Climatic factors, morphological characters, and soil variables were measured and analyzed to provide additional support for recovered groups. Results: The STACEY analysis recovered three major clades and seven subclades, all of which are geographically structured, and some correspond to previously named taxa. No other analysis had full agreement between recovered clades and other parameters. Climatic niche and leaflet width and length provide some predictive ability for the major clades. Conclusions: The results suggest that these groups are in the process of incipient speciation and incomplete lineage sorting has been a major barrier to resolving boundaries within this lineage previously. These results are hypothesized through sequencing of multiple loci and analyzing data using coalescent-based processes.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): Angiosperms353, coalescence, incipient speciation, incomplete lineage sorting, incongruence, STACEY
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Research Centres and Institutes: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Steven Dodsworth
    Date Deposited: 26 Sep 2024 09:22
    Last Modified: 26 Sep 2024 15:56
    URI: https://eprints.bbk.ac.uk/id/eprint/54034

    Statistics

    Activity Overview
    6 month trend
    6Downloads
    6 month trend
    19Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item
    Edit/View Item