Pyle, Euan and Miller, E.A. and Zanetti, Giulia (2025) Cryo-electron tomography reveals how COPII assembles on cargo-containing membranes. Nature Structural & Molecular Biology 32 , pp. 513-519. ISSN 1545-9993.
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Abstract
Proteins traverse the eukaryotic secretory pathway through membrane trafficking between organelles. The coat protein complex II (COPII) mediates the anterograde transport of newly synthesized proteins from the endoplasmic reticulum, engaging cargoes with a wide range of size and biophysical properties. The native architecture of the COPII coat and how cargo might influence COPII carrier morphology remain poorly understood. Here we reconstituted COPII-coated membrane carriers using purified Saccharomyces cerevisiae proteins and cell-derived microsomes as a native membrane source. Using cryo-electron tomography with subtomogram averaging, we demonstrate that the COPII coat binds cargo and forms largely spherical vesicles from native membranes. We reveal the architecture of the inner and outer coat layers and shed light on how spherical carriers are formed. Our results provide insights into the architecture and regulation of the COPII coat and advance our current understanding of how membrane curvature is generated.
Metadata
| Item Type: | Article |
|---|---|
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Depositing User: | Giulia Zanetti |
| Date Deposited: | 08 May 2025 13:15 |
| Last Modified: | 15 Jul 2025 11:07 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/55542 |
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