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    Cryo-electron tomography reveals how COPII assembles on cargo-containing membranes

    Pyle, Euan and Miller, E.A. and Zanetti, Giulia (2025) Cryo-electron tomography reveals how COPII assembles on cargo-containing membranes. Nature Structural & Molecular Biology 32 , pp. 513-519. ISSN 1545-9993.

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    Abstract

    Proteins traverse the eukaryotic secretory pathway through membrane trafficking between organelles. The coat protein complex II (COPII) mediates the anterograde transport of newly synthesized proteins from the endoplasmic reticulum, engaging cargoes with a wide range of size and biophysical properties. The native architecture of the COPII coat and how cargo might influence COPII carrier morphology remain poorly understood. Here we reconstituted COPII-coated membrane carriers using purified Saccharomyces cerevisiae proteins and cell-derived microsomes as a native membrane source. Using cryo-electron tomography with subtomogram averaging, we demonstrate that the COPII coat binds cargo and forms largely spherical vesicles from native membranes. We reveal the architecture of the inner and outer coat layers and shed light on how spherical carriers are formed. Our results provide insights into the architecture and regulation of the COPII coat and advance our current understanding of how membrane curvature is generated.

    Metadata

    Item Type: Article
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Depositing User: Giulia Zanetti
    Date Deposited: 08 May 2025 13:15
    Last Modified: 14 Jun 2025 17:29
    URI: https://eprints.bbk.ac.uk/id/eprint/55542

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