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    Helical ordering of envelope-associated proteins and glycoproteins in respiratory syncytial virus

    Conley, M.J. and Short, J.M. and Burns, A.M. and Streetley, J. and Hutchings, J. and Bakker, S.E. and Power, B.J. and Jaffery, H. and Haney, J. and Zanetti, Giulia and Murcia, P.R. and Stewart, M. and Fearns, R. and Vijayakrishnan, S. and Bhella, D. (2021) Helical ordering of envelope-associated proteins and glycoproteins in respiratory syncytial virus. The EMBO Journal 41 (3), ISSN 0261-4189.

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    Abstract

    Human respiratory syncytial virus (RSV) causes severe respiratory illness in children and the elderly. Here, using cryogenic electron microscopy and tomography combined with computational image analysis and three‐dimensional reconstruction, we show that there is extensive helical ordering of the envelope‐associated proteins and glycoproteins of RSV filamentous virions. We calculated a 16 Å resolution sub‐tomogram average of the matrix protein (M) layer that forms an endoskeleton below the viral envelope. These data define a helical lattice of M‐dimers, showing how M is oriented relative to the viral envelope. Glycoproteins that stud the viral envelope were also found to be helically ordered, a property that was coordinated by the M‐layer. Furthermore, envelope glycoproteins clustered in pairs, a feature that may have implications for the conformation of fusion (F) glycoprotein epitopes that are the principal target for vaccine and monoclonal antibody development. We also report the presence, in authentic virus infections, of N‐RNA rings packaged within RSV virions. These data provide molecular insight into the organisation of the virion and the mechanism of its assembly.

    Metadata

    Item Type: Article
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Depositing User: Giulia Zanetti
    Date Deposited: 08 May 2025 13:03
    Last Modified: 14 Jun 2025 08:39
    URI: https://eprints.bbk.ac.uk/id/eprint/55545

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