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    Structure of a translocation signal domain mediating conjugative transfer by Type IV secretion systems

    Redzej, Adam and Ilangovan, Aravindan and Lang, S. and Gruber, C.J. and Topf, Maya and Zangger, K. and Zechner, E.L. and Waksman, Gabriel (2013) Structure of a translocation signal domain mediating conjugative transfer by Type IV secretion systems. Molecular Microbiology 89 (2), pp. 324-333. ISSN 0950-382X.

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    Relaxases are proteins responsible for the transfer of plasmid and chromosomal DNA from one bacterium to another during conjugation. They covalently react with a specific phosphodiester bond within DNA origin of transfer sequences, forming a nucleo-protein complex which is subsequently recruited for transport by a plasmid-encoded type IV secretion system. In previous work we identified the targeting translocation signals presented by the conjugative relaxase TraI of plasmid R1. Here we report the structure of TraI translocation signal TSA. In contrast to known translocation signals we show that TSA is an independent folding unit and thus forms a bona fide structural domain. This domain can be further divided into three sub-domains with striking structural homology with helicase sub-domains of the SF1B family. We also show that TSA is part of a larger vestigial helicase domain which has lost its helicase activity but not its single-stranded DNA binding capability. Finally, we further delineate the binding site responsible for translocation activity of TSA by targeting single residues for mutations. Overall, this study provides the first evidence that translocation signals can be part of larger structural scaffolds, overlapping with translocation-independent activities.


    Item Type: Article
    Keyword(s) / Subject(s): translocation signal SF1B helicase, TraI, relaxase
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Research Centres and Institutes: Bioinformatics, Bloomsbury Centre for (Closed), Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 29 May 2013 11:54
    Last Modified: 02 Aug 2023 17:04


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