BIROn - Birkbeck Institutional Research Online

    Circular and linear dichroism spectroscopy for the study of protein–ligand interactions

    Daviter, Tina and Chmel, N. and Rodger, A. (2013) Circular and linear dichroism spectroscopy for the study of protein–ligand interactions. In: Williams, Mark A. and Daviter, Tina (eds.) Protein–Ligand Interactions: Fundamentals. Methods in Molecular Biology III 1008. Berlin, Germany: Springer, pp. 211-241. ISBN 9781627033978.

    Full text not available from this repository.


    Circular dichroism (CD) is the difference in absorption of left and right circularly polarized light, usually by a solution containing the molecules of interest. A non-zero signal for solutions is only measured for chiral molecules such as proteins whose mirror image is not superposable on the original molecule. A CD spectrum provides information about the bonds and structures responsible for the chirality. When a small molecule (or ligand) binds to a protein, it acquires an induced CD (ICD) spectrum through chiral perturbation to its structure or electron rearrangements (transitions). The wavelengths of this ICD are determined by the ligand’s own absorption spectrum, and the intensity of the ICD spectrum is determined by the strength and geometry of its interaction with the protein. Thus, ICD can be used to probe the binding of ligands to proteins. This chapter contains an outline of how to perform protein CD and ICD experiments, together with some of the issues relating to experimental design and implementation. Addition of a quarter wave plate to a CD spectropolarimeter converts it to a linear dichroism (LD) spectrometer. When protein samples are aligned either in flow (as for fibers or membrane proteins in liposomes) or on surfaces the orientations of ligands with respect to the protein backbone or other subunits can be determined.


    Item Type: Book Section
    Additional Information: Series ISSN: 1064-3745
    Keyword(s) / Subject(s): Circular dichroism, Proteins, Chirality, Ligand binding, Induced circular dichroism, Linear dichroism
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Depositing User: Administrator
    Date Deposited: 29 Jan 2014 13:36
    Last Modified: 02 Aug 2023 17:09


    Activity Overview
    6 month trend
    6 month trend

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item