Identification of a key residue for Oligomerisation and pore-formation of Clostridium perfringens NetB
da Costa, S. and Savva, Christos G. and Bokori-Brown, M. and Naylor, Claire E. and Moss, David S. and Basak, Ajit K. and Titball, R. (2014) Identification of a key residue for Oligomerisation and pore-formation of Clostridium perfringens NetB. Toxins 6 (3), pp. 1049-1061. ISSN 2072-6651.
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Abstract
Necrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant economic damage to the poultry industry worldwide. In this study, site-directed mutagenesis was used to identify amino acids that play a role in NetB oligomerisation and pore-formation. NetB K41H showed significantly reduced toxicity towards LMH cells and human red blood cells relative to wild type toxin. NetB K41H was unable to oligomerise and form pores in liposomes. These findings suggest that NetB K41H could be developed as a genetic toxoid vaccine to protect against necrotic enteritis.
Metadata
| Item Type: | Article |
|---|---|
| Keyword(s) / Subject(s): | NetB, pore-forming toxin, Clostridium perfringens, necrotic enteritis |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Research Centres and Institutes: | Structural Molecular Biology, Institute of (ISMB) |
| Depositing User: | Administrator |
| Date Deposited: | 18 Mar 2014 15:18 |
| Last Modified: | 02 Aug 2023 17:09 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/9367 |
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