Pinheiro, Vitor B. and Arangundy-Franklin, S. and Holliger, P. (2014) Compartmentalized self-tagging for in vitro-directed evolution of XNA polymerases. Current Protocols In Nucleic Acid Chemistry 57 , 9.9.1-9.9.18.
Abstract
Template-dependent synthesis of xenobiotic nucleic acids (XNAs) is an essential step for the development of functional XNA molecules, as it enables Darwinian evolution to be carried out with novel genetic polymers. The extraordinary substrate specificity of natural DNA polymerases greatly restricts the spectrum of XNAs available, thus making it necessary to identify DNA polymerase variants capable of incorporating a wider range of substrates. This unit summarizes compartmentalized self-tagging (CST), a directed evolution strategy developed for the selection of DNA polymerase variants capable of XNA synthesis.
Metadata
| Item Type: | Article |
|---|---|
| Additional Information: | ISBN: 9780471142706 |
| Keyword(s) / Subject(s): | DNA polymerases, in vitro-directed evolution, synthetic nucleic acids, XNA, compartmentalized self-tagging, CST |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Research Centres and Institutes: | Structural Molecular Biology, Institute of (ISMB) |
| Depositing User: | Administrator |
| Date Deposited: | 01 Jul 2014 09:11 |
| Last Modified: | 02 Aug 2023 17:11 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/10040 |
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