Rizi, K. and Murdan, S. and Danquah, Cynthia A. and Nicklin, Jane and Bhakta, Sanjib (2015) Development of a rapid, reliable and quantitative method – “SPOTi” for testing antifungal efficacy. Journal of Microbiological Methods 117 , pp. 36-40. ISSN 0167-7012.
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Abstract
A reference method for the antimicrobial susceptibility testing of common fungal pathogens such as dermatophytes, is currently lacking. In this study, we report the successful adaptation of solid agar-based spot culture growth inhibition assay (SPOTi) for dermatophytes, currently being used as a gold-standard in the anti-tubercular drug discovery field. The fungal-SPOTi assay correlated with the disc-diffusion method, and is validated using mycelial plugs. We propose the fungal-SPOTi as a high-throughput alternative to the disc-diffusion and broth micro-dilution anti-fungal assays to screen novel anti-fungals.
Metadata
| Item Type: | Article |
|---|---|
| Keyword(s) / Subject(s): | SPOTi, antifungals, dermatophytes, screening, drug discovery |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Research Centres and Institutes: | Structural Molecular Biology, Institute of (ISMB) |
| Depositing User: | Administrator |
| Date Deposited: | 22 Jul 2015 12:38 |
| Last Modified: | 02 Aug 2023 17:17 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/12552 |
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