Naylor, Claire and Bagneris, Claire and DeCaen, P.G. and Sula, A. and Scaglione, A. and Clapham, D.E. and Wallace, Bonnie A. (2016) Molecular basis of ion permeability in a voltage-gated sodium channel. Embo Journal 35 (8), pp. 820-830. ISSN 0261-4189.
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Abstract
Voltage‐gated sodium channels are essential for electrical signalling across cell membranes. They exhibit strong selectivities for sodium ions over other cations, enabling the finely tuned cascade of events associated with action potentials. This paper describes the ion permeability characteristics and the crystal structure of a prokaryotic sodium channel, showing for the first time the detailed locations of sodium ions in the selectivity filter of a sodium channel. Electrostatic calculations based on the structure are consistent with the relative cation permeability ratios (Na+ ≈ Li+ ≫ K+, Ca2+, Mg2+) measured for these channels. In an E178D selectivity filter mutant constructed to have altered ion selectivities, the sodium ion binding site nearest the extracellular side is missing. Unlike potassium ions in potassium channels, the sodium ions in these channels appear to be hydrated and are associated with side chains of the selectivity filter residues, rather than polypeptide backbones.
Metadata
| Item Type: | Article |
|---|---|
| Keyword(s) / Subject(s): | crystal structure, electrophysiology, ion permeability, sodium channel |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Research Centres and Institutes: | Bioinformatics, Bloomsbury Centre for (Closed), Structural Molecular Biology, Institute of (ISMB) |
| Depositing User: | Administrator |
| Date Deposited: | 04 Mar 2016 11:23 |
| Last Modified: | 02 Aug 2023 17:22 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/14618 |
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