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    Delineation of the TRAK binding regions of the kinesin-1 motor proteins

    Randall, T.S. and Moores, Carolyn A. and Stephenson, F.A. (2013) Delineation of the TRAK binding regions of the kinesin-1 motor proteins. FEBS Letters 587 (23), pp. 3763-3769. ISSN 1873-3468.

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    Abstract

    Understanding specific cargo distribution in differentiated cells is a major challenge. Trafficking kinesin proteins (TRAKs) are kinesin adaptors. They bind the cargo binding domain of kinesin-1 motor proteins forming a link between the motor and their cargoes. To refine the TRAK1/2 binding sites within the kinesin-1 cargo domain, rationally designed C-terminal truncations of KIF5A and KIF5C were generated and their co-association with TRAK1/2 determined by quantitative co-immunoprecipitations following co-expression in mammalian cells. Three contributory regions forming the TRAK2 binding site within KIF5A and KIF5C cargo binding domains were delineated. Differences were found between TRAK1/2 with respect to association with KIF5A.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): kinesin, kinesin adaptor proteins, TRAK, motor proteins, intracellular transport, mitochondrial trafficking
    School: School of Science > Biological Sciences
    Research Centres and Institutes: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Carolyn Moores
    Date Deposited: 23 Jun 2016 13:23
    Last Modified: 12 Jun 2021 13:28
    URI: https://eprints.bbk.ac.uk/id/eprint/15610

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