Goosens, V.J. and Busch, Andreas and Georgiadou, M. and Castagnini, M. and Forest, K.T. and Waksman, Gabriel and Pelicic, V. (2017) Reconstitution of a minimal machinery capable of assembling periplasmic type IV pili. Proceedings of the National Academy of Sciences of the United States of America 114 (25), E4978-E4986. ISSN 0027-8424.
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Abstract
Type IV pili (Tfp), which are key virulence factors in many bacterial pathogens, define a large group of multipurpose filamentous nanomachines widespread in Bacteria and Archaea. Tfp biogenesis is a complex multistep process, which relies on macromolecular assemblies composed of 15 conserved proteins in model gram- negative species. To improve our limited understanding of the molecular mechanisms of filament assembly, we have used a synthetic biology approach to reconstitute, in a nonnative heter- ologous host, a minimal machinery capable of building Tfp. Here we show that eight synthetic genes are sufficient to promote filament assembly and that the corresponding proteins form a macromolecular complex at the cytoplasmic membrane, which we have purified and characterized biochemically. Our results con- tribute to a better mechanistic understanding of the assembly of remarkable dynamic filaments nearly ubiquitous in prokaryotes.
Metadata
| Item Type: | Article |
|---|---|
| Keyword(s) / Subject(s): | type IV pili, type IV filamentous nanomachines, filament assembly, synthetic biology |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Depositing User: | Administrator |
| Date Deposited: | 14 Aug 2017 13:29 |
| Last Modified: | 02 Aug 2023 17:34 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/19382 |
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