Sissons, J. and Kim, K.S. and Stins, M. and Jayasekera, S. and Alsam, S. and Khan, Naveed Ahmed (2005) Acanthamoeba castellanii induces host cell death via a phosphatidylinositol 3-kinase-dependent mechanism. Infection and Immunity 73 (5), pp. 2704-2708. ISSN 0019-9567.
|
Text
khan6.pdf Download (109kB) | Preview |
Abstract
Granulomatous amoebic encephalitis due to Acanthamoeba castellanii is a serious human infection with fatal consequences, but it is not clear how the circulating amoebae interact with the blood-brain barrier and transmigrate into the central nervous system. We studied the effects of an Acanthamoeba encephalitis isolate belonging to the T1 genotype on human brain microvascular endothelial cells, which constitute the blood-brain barrier. Using an apoptosis-specific enzyme-linked immunosorbent assay, we showed that Acanthamoeba induces programmed cell death in brain microvascular endothelial cells. Next, we observed that Acanthamoeba specifically activates phosphatidylinositol 3-kinase. Acanthamoeba-mediated brain endothelial cell death was abolished using LY294002, a phosphatidylinositol 3-kinase inhibitor. These results were further confirmed using brain microvascular endothelial cells expressing dominant negative forms of phosphatidylinositol 3-kinase. This is the first demonstration that Acanthamoeba-mediated brain microvascular endothelial cell death is dependent on phosphatidylinositol 3-kinase.
Metadata
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright © 2005, American Society for Microbiology. All Rights Reserved. |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Depositing User: | Administrator |
| Date Deposited: | 17 Aug 2005 |
| Last Modified: | 02 Aug 2023 16:46 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/203 |
Statistics
Additional statistics are available via IRStats2.
Archive Staff Only (login required)
