Legionella DotM structure reveals a role in effector recruiting to the Type 4B secretion system

Meir, A. and Chetrit, D. and Liu, L. and Roy, C. and Waksman, Gabriel (2018) Legionella DotM structure reveals a role in effector recruiting to the Type 4B secretion system. Nature Communications 9 , p. 507. ISSN 2041-1723.

Abstract

Legionella pneumophila, a causative agent of pneumonia, utilizes the Type 4B secretion (T4BS) system to translocate over 300 effectors into the host cell during infection. T4BS systems are encoded by a large gene cluster termed dot/icm, three components of which, DotL, DotM, and DotN, form the “coupling complex”, which serves as a platform for recruitment of effector proteins. One class of effectors includes proteins containing Glu-rich/E-block sequences at their C terminus. However, the protein or region of the coupling complex mediating recruitment of such effectors is unknown. Here we present the crystal structure of DotM. This all alpha-helical structure exhibits patches of positively charged residues. We show that these regions form binding sites for acidic Glu-rich peptides and that mutants targeting these patches are defective in vivo in the translocation of acidic Glu-rich motif-containing effectors. We conclude that DotM forms the interacting surface for recruitment of acidic Glu-rich motif-containing Legionella effectors.

Metadata

Statistics

DownloadsShow export options

Export as XMLJSONCSV

Activity Overview

6 month trend

269Downloads

6 month trend

145Hits

Additional statistics are available via IRStats2.

Archive Staff Only (login required)

Edit/View Item