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    Legionella DotM structure reveals a role in effector recruiting to the Type 4B secretion system

    Meir, A. and Chetrit, D. and Liu, L. and Roy, C. and Waksman, Gabriel (2018) Legionella DotM structure reveals a role in effector recruiting to the Type 4B secretion system. Nature Communications 9 , p. 507. ISSN 2041-1723.

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    Abstract

    Legionella pneumophila, a causative agent of pneumonia, utilizes the Type 4B secretion (T4BS) system to translocate over 300 effectors into the host cell during infection. T4BS systems are encoded by a large gene cluster termed dot/icm, three components of which, DotL, DotM, and DotN, form the “coupling complex”, which serves as a platform for recruitment of effector proteins. One class of effectors includes proteins containing Glu-rich/E-block sequences at their C terminus. However, the protein or region of the coupling complex mediating recruitment of such effectors is unknown. Here we present the crystal structure of DotM. This all alpha-helical structure exhibits patches of positively charged residues. We show that these regions form binding sites for acidic Glu-rich peptides and that mutants targeting these patches are defective in vivo in the translocation of acidic Glu-rich motif-containing effectors. We conclude that DotM forms the interacting surface for recruitment of acidic Glu-rich motif-containing Legionella effectors.

    Metadata

    Item Type: Article
    School: School of Science > Biological Sciences
    Depositing User: Administrator
    Date Deposited: 12 Feb 2018 10:14
    Last Modified: 09 Feb 2021 17:13
    URI: https://eprints.bbk.ac.uk/id/eprint/21028

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