Hutchings, J. and Zanetti, Giulia (2018) Fine details in complex environments: the power of cryo-electron tomography. Biochemical Society Transactions , ISSN 0300-5127.
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Abstract
Cryo-electron tomography (CET) is uniquely suited to obtain structural information from a wide range of biological scales, integrating and bridging knowledge from molecules to cells. In particular, CET can be used to visualise molecular structures in their native environment. Depending on the experiment, a varying degree of resolutions can be achieved, with the first near-atomic molecular structures becoming recently available. The power of CET has increased significantly in the last 5 years, in parallel with improvements in cryo-EM hardware and software that have also benefited single-particle reconstruction techniques. In this review, we cover the typical CET pipeline, starting from sample preparation, to data collection and processing, and highlight in particular the recent developments that support structural biology We provide some examples that highlight the importance of structure determination of molecules embedded within their native environment, and propose future directions to improve CET performance and accessibility. [Abstract copyright: © 2018 The Author(s).]
Metadata
| Item Type: | Article |
|---|---|
| Keyword(s) / Subject(s): | cryo-electron microscopy, cryo-electron tomography, subtomogram averaging |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| SWORD Depositor: | Mr Joe Tenant |
| Depositing User: | Giulia Zanetti |
| Date Deposited: | 04 Jul 2018 12:51 |
| Last Modified: | 02 Aug 2023 17:42 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/23022 |
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