Trokter, Martina and Waksman, Gabriel (2018) Translocation through the Conjugative Type IV Secretion System requires unfolding of its protein substrate. Journal of Bacteriology 200 (20), ISSN 0021-9193.
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Abstract
Bacterial conjugation, a mechanism of horizontal gene transfer, is the major means by which antibiotic resistance spreads among bacteria (1, 2). Conjugative plasmids are transferred from one bacterium to another through a type IV secretion system (T4SS) in the form of single-stranded DNA covalently attached to a protein called relaxase. The relaxase is fully functional both in a donor cell (prior to conjugation) and recipient cell (after conjugation). Here, we demonstrate that the protein substrate has to unfold for efficient translocation through the conjugative T4SS. Furthermore, we present various relaxase modifications that preserve the function of the relaxase but block substrate translocation. This study brings us a step closer to deciphering the complete mechanism of T4SS substrate translocation, which is vital for the development of new therapies against multidrug-resistant pathogenic bacteria.
Metadata
| Item Type: | Article |
|---|---|
| Keyword(s) / Subject(s): | T4SS, transport, conjugation, secretion systems, type 4 secretion systems, unfolding |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| SWORD Depositor: | Mr Joe Tenant |
| Depositing User: | Mr Joe Tenant |
| Date Deposited: | 07 Nov 2018 14:42 |
| Last Modified: | 02 Aug 2023 17:44 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/24558 |
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