Mylona, Anastasia and Carr, S. and Aller, P. and Moraes, I. and Treisman, R. and Evans, G. and Foadi, J. (2017) A novel approach to data collection for difficult structures: data management for large numbers of crystals with the BLEND software. Crystals 7 (8), p. 242. ISSN 2073-4352.
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Abstract
The present article describes how to use the computer program BLEND to help assemble complete datasets for the solution of macromolecular structures, starting from partial or complete datasets, derived from data collection from multiple crystals. The program is demonstrated on more than two hundred X-ray diffraction datasets obtained from 50 crystals of a complex formed between the SRF transcription factor, its cognate DNA, and a peptide from the SRF cofactor MRTF-A. This structure is currently in the process of being fully solved. While full details of the structure are not yet available, the repeated application of BLEND on data from this structure, as they have become available, has made it possible to produce electron density maps clear enough to visualise the potential location of MRTF sequences.
Metadata
| Item Type: | Article |
|---|---|
| Keyword(s) / Subject(s): | BLEND, multiple crystals, hierarchical cluster analysis, protein-protein-DNA complex |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Depositing User: | Administrator |
| Date Deposited: | 13 Nov 2018 14:49 |
| Last Modified: | 02 Aug 2023 17:46 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/25123 |
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