BIROn - Birkbeck Institutional Research Online

    A novel approach to data collection for difficult structures: data management for large numbers of crystals with the BLEND software

    Mylona, Anastasia and Carr, S. and Aller, P. and Moraes, I. and Treisman, R. and Evans, G. and Foadi, J. (2017) A novel approach to data collection for difficult structures: data management for large numbers of crystals with the BLEND software. Crystals 7 (8), p. 242. ISSN 2073-4352.

    [img]
    Preview
    Text
    25123.pdf - Published Version of Record
    Available under License Creative Commons Attribution.

    Download (2MB) | Preview

    Abstract

    The present article describes how to use the computer program BLEND to help assemble complete datasets for the solution of macromolecular structures, starting from partial or complete datasets, derived from data collection from multiple crystals. The program is demonstrated on more than two hundred X-ray diffraction datasets obtained from 50 crystals of a complex formed between the SRF transcription factor, its cognate DNA, and a peptide from the SRF cofactor MRTF-A. This structure is currently in the process of being fully solved. While full details of the structure are not yet available, the repeated application of BLEND on data from this structure, as they have become available, has made it possible to produce electron density maps clear enough to visualise the potential location of MRTF sequences.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): BLEND, multiple crystals, hierarchical cluster analysis, protein-protein-DNA complex
    School: School of Science > Biological Sciences
    Depositing User: Administrator
    Date Deposited: 13 Nov 2018 14:49
    Last Modified: 24 Jun 2020 16:28
    URI: https://eprints.bbk.ac.uk/id/eprint/25123

    Statistics

    Downloads
    Activity Overview
    153Downloads
    52Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item