Cryo-EM structure (4.5-Å) of Yeast Kinesin-5–Microtubule Complex reveals a distinct binding footprint and mechanism of drug resistance

von Loeffelholz, O. and Peña, A. and Drummond, D.R. and Cross, R. and Moores, Carolyn A. (2019) Cryo-EM structure (4.5-Å) of Yeast Kinesin-5–Microtubule Complex reveals a distinct binding footprint and mechanism of drug resistance. Journal of Molecular Biology 431 (4), pp. 864-872. ISSN 0022-2836.

Abstract

Kinesin-5s are microtubule-dependent motors that drive spindle pole separation during mitosis. We used cryo-electron microscopy to determine the 4.5 Å resolution structure of the motor domain of the fission yeast kinesin-5 Cut7 bound to fission yeast microtubules, and explored the topology of the motor-microtubule interface and the susceptibility of the complex to drug binding. Despite their non-canonical architecture and mechanochemistry, S. pombe microtubules were stabilized by epothilone at the taxane binding pocket. The overall Cut7 footprint on the S. pombe microtubule surface is altered compared to mammalian tubulin microtubules because of their different polymer architectures. However, the core motor-microtubule interaction is tightly conserved, reflected in similar Cut7 ATPase activities on each microtubule type. AMPPNP-bound Cut7 adopts a kinesin-conserved ATP-like conformation including cover neck bundle formation. However, the Cut7 ATPase is not blocked by a mammalian-specific kinesin-5 inhibitor, consistent with the non-conserved sequence and structure of its loop5 insertion.

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